akt family and mirnas expression in il-2-induced cd4+t cells

objective(s): study of non-coding rnas is considerable to elucidate principal biological questions or design new therapeutic strategies. mirnas are a group of non-coding rnas that their functions in pi3k/akt signaling and apoptosis pathways after t cell activation is not entirely clear. herein, mirnas expression and their putative targets in the mentioned pathways were studied in the activated cd4+t cells. materials and methods: herein, proliferation rate and il-2 secretion were measured in treated and untreated cells by il-2. putative targets of up-regulated mirnas were predicted by bioinformatics approaches in the apoptotic and pi3k/akt signaling pathways. then the expression of two putative targets was evaluated by quantitative rt-pcr.  results: proliferation rate of treated cells by il-2 increased in a dose- and time- dependent manner. naive and activated cd4+t cells  induced by different dose of il-2 secreted abundant amounts of il-2. also, in il-2 un-induced cells (il-2 depleted cells) after 3 days, decrease of proliferation has been shown. in silico analysis predicted putative targets of up-regulated mirnas such as akt1, akt3 and apoptotic genes in the activated cells induced or un-induced by il-2. decrease of akt3 was shown by q-rt-pcr as a potential target of mirnas overexpressed in il-2 depleted cells. but there was no significant difference in akt1 expression in two cell groups. conclusion:  our analysis suggests that decrease of akt3 was likely controlled via up-regulation of specific mirnas in il-2 depleted cells. also it seems that mirnas play role in induction of different apoptosis pathways in il-2 induced and un-induced cells.